| Samuel Allyne Otis | 17890408_18140422Apr 8, 1789–Apr 22, 1814 | Otis died on April 22, 1814, just four days after the end of the second session of the 13th Congress. When the Senate convened a third session of the 13th Congress on September 19, 1814, it swore into office Chief Clerk Samuel Turner, Jr., as acting secretary. Turner served until Charles Cutts took office the following month. |
| Charles Cutts | 18141012_18251212Oct 12, 1814–Dec 12, 1825 | – |
| Walter Lowrie | 18251212_18361205Dec 12, 1825–Dec 5, 1836 | – |
| Asbury Dickins | 18361213_18610715Dec 13, 1836–Jul 15, 1861 | – |
| John W. Forney | 18610715_18680604Jul 15, 1861–Jun 4, 1868 | – |
| George C. Gorham | 18680606_18790324Jun 6, 1868–Mar 24, 1879 | – |
| John C. Burch | 18790324_18810728Mar 24, 1879–Jul 28, 1881 | After Burch died in office, Francis E. Shober was elected acting secretary during a special session of the Senate in October 1881. When the 47th Congress convened in December 1881, the Senate was evenly divided with 37 Democrats and 37 Republicans, and they deadlocked over the election of officers. Shober continued as acting secretary for two more years until the 48th Congress, dominated by Republicans, elected a new secretary. |
| Anson G. McCook | 18831218_18930807Dec 18, 1883–Aug 7, 1893 | – |
| William Ruffin Cox | 18930807_19000131Aug 7, 1893–Jan 31, 1900 | – |
| Charles G. Bennett | 19000201_19130313Feb 1, 1900–Mar 13, 1913 | – |
| James M. Baker | 19130313_19130519Mar 13, 1913–May 19, 1919 | – |
| George A. Sanderson | 19190519_19250424May 19, 1919–Apr 24, 1925 | – |
| Edwin Pope Thayer | 19251207_19330309Dec 7, 1925–Mar 9, 1933 | – |
| Edwin A. Halsey | 19330309_19450129Mar 9, 1933–Jan 29, 1945 | – |
| Leslie Biffle | 19450208_19470104Feb 8, 1945–Jan 4, 1947 | Leslie Biffle was first elected acting secretary after Halsey's death, and then—about a week later—was chosen secretary by a subsequent resolution of the Senate. |
| Carl A. Loeffler | 19450208_19470104Jan 4, 1947 –Jan 3, 1949 | – |
| Leslie Biffle | 19490103_19530103Jan 3, 1949 – Jan 3, 1953 | – |
| J. Mark Trice | 19530103_19550105Jan 3, 1953–Jan 5, 1955 | – |
| Felton M. Johnston | 19550105_19651230Jan 5, 1955–Dec 30, 1965 | – |
| Emery L. Frazier | 19660101_19660930Jan 1, 1966–Sept 30, 1966 | – |
| Francis R. Valeo | 19661001_19770331Oct 1, 1966–Mar 31, 1977 | Valeo was elected on August 20, 1965 by a resolution of the Senate which specified that his term commence on October 1, 1966 (Senate Resolution 140, 89th Congress, 1st session). He was sworn in on September 30, 1966. |
| J. Stanley Kimmitt | 19770401_19810104Apr 1, 1977–Jan 4, 1981 | – |
| William F. Hildenbrand | 19810105_19850102Jan 5, 1981–Jan 2, 1985 | – |
| Jo–Anne L. Coe | 19850103_19870106Jan 3, 1985–Jan 6, 1987 | – |
| Walter J. Stewart | 19870106_19940415Jan 6, 1987–Apr 15, 1994 | – |
| Martha S. Pope | 19940415_19950103Apr 15, 1994–Jan 3, 1995 | – |
| Sheila P. Burke | 19950104_19950607Jan 4, 1995–Jun 7, 1995 | – |
| Kelly D. Johnston | 19950608_19960930Jun 8, 1995–Sept 30, 1996 | – |
| Gary Lee Sisco | 19961001_20010711Oct 1, 1996–Jul 11, 2001 | – |
| Jeri Thomson | 20010712_20030106Jul 12, 2001–Jan 6, 2003 | – |
| Emily J. Reynolds | 20030107_20070104Jan 7, 2003–Jan 4, 2007 | – |
| Nancy Erickson | 20070104_20150106Jan 4, 2007–Jan 6, 2015 | – |
| Julie E. Adams | 20150106Jan 6, 2015–Mar 1, 2021 | – |
| Sonceria Ann Berry | 20210301Mar 1, 2021–Jan 3, 2025 | – |
| Jackie Barber | 20250103Jan 3, 2025–present | – |
