| CONGRESS | SENATOR | TERM BEGAN | TERM ENDED | COMMENTS |
|---|---|---|---|---|
| Class 1 | ||||
| 29th-31st | David Levy Yulee1 (D) | Jul. 1, 1845 | Mar. 3, 1851 | |
| 32nd-36th | Stephen R. Mallory (D) | Mar. 4, 1851 | Jan. 21, 18612 | Withdrew |
| 37th-40th | Adonijah S. Welch (D) | Jun. 25, 18683 | Mar. 3, 1869 | |
| 41st-43rd | Abijah Gilbert (R) | Mar. 4, 1869 | Mar. 3, 1875 | |
| 44th-49th | Charles W. Jones (D) | Mar. 4, 1875 | Mar. 3, 1887 | |
| 50th-55th | Samuel Pasco4 (D) | May 19, 1887 | Apr. 18, 1899 | |
| 55th-61st | James P. Taliaferro (D) | Apr. 20, 18995 | Mar. 3, 1911 | |
| 62nd-64th | Nathan P. Bryan (D) | Mar. 4, 1911 | Mar. 3, 1917 | Appointed, then elected |
| 65th-74th | Park Trammell (D) | Mar. 4, 1917 | May 8, 1936 | Died |
| 74th | Scott M. Loftin (D) | May 26, 1936 | Nov. 3, 1936 | Appointed |
| 74th-79th | Charles O. Andrews (D) | Nov. 4, 1936 | Sept. 18, 1946 | Died |
| 79th-91st | Spessard L. Holland (D) | Sept. 25, 1946 | Jan. 3, 1971 | Appointed, then elected |
| 92nd-100th | Lawton M. Chiles Jr. (D) | Jan. 3, 1971 | Jan. 3, 1989 | |
| 101st-106th | Connie Mack III (R) | Jan. 3, 1989 | Jan. 3, 2001 | |
| 107th-115th | Clarence William (Bill) Nelson (D) | Jan. 3, 2001 | Jan. 3, 2019 | |
| 116th- | Rick Scott (R) | Jan. 8, 20196 | Present | |
| Class 3 | ||||
| 29th-30th | James D. Westcott Jr. (D) | Jul. 1, 1845 | Mar. 3, 1849 | |
| 31st-33rd | Jackson Morton (W) | Mar. 4, 1849 | Mar. 3, 1855 | |
| 34th-36th | David Levy Yulee (D) | Mar. 4, 1855 | Jan. 21, 1861 | Withdrew |
| 40th-42nd | Thomas W. Osborn (R) | Jun. 25, 18687 | Mar. 3, 1873 | |
| 43rd-45th | Simon B. Conover (R) | Mar. 4, 1873 | Mar. 3, 1879 | |
| 46th-54th | Wilkinson Call (D) | Mar. 4, 1879 | Mar. 3, 1897 | |
| 55th-60th | Stephen R. Mallory (D) | May 15, 18978 | Dec. 23, 1907 | Appointed, then elected; died |
| 60th | William J. Bryan (D) | Dec. 26, 1907 | Mar. 22, 1908 | Appointed; died |
| 60th | William H. Milton (D) | Mar. 27, 1908 | Mar. 3, 1909 | Appointed |
| 61st-74th | Duncan U. Fletcher (D) | Mar. 4, 1909 | Jun. 17, 1936 | Appointed, then elected; died |
| 74th | William L. Hill (D) | Jul. 1, 1936 | Nov. 3, 1936 | Appointed |
| 74th-81st | Claude D. Pepper (D) | Nov. 4, 1936 | Jan. 3, 1951 | |
| 82nd-90th | George A. Smathers (D) | Jan. 3, 1951 | Jan. 3, 1969 | |
| 91st-93rd | Edward J. Gurney (R) | Jan. 3, 1969 | Dec. 31, 1974 | Resigned |
| 93rd-96th | Richard B. Stone (D) | Jan. 1, 19759 | Dec. 31, 1980 | Resigned |
| 96th-99th | Paula Hawkins (R) | Jan. 1, 198110 | Jan. 3, 1987 | |
| 100th-108th | Daniel Robert (Bob) Graham (D) | Jan. 3, 1987 | Jan. 3, 2005 | |
| 109th-111th | Melquiades R. (Mel) Martinez (R) | Jan. 3, 2005 | Sept. 9, 2009 | Resigned |
| 111th | George S. LeMieux (R) | Sept. 10, 2009 | Jan. 3, 2011 | Appointed |
| 112th-119th | Marco Rubio (R) | Jan. 3, 2011 | Jan. 20, 202511 | Resigned |
| 119th- | Ashley Moody (R) | Jan. 21, 2025 | Present | Appointed |
1. Elected as David Levy. Changed name to David Levy Yulee in 1846.
2. Withdrew from the Senate on Jan. 21, 1861. Seat declared vacant on Mar. 4, 1861.
3. Seat vacant from Mar. 4, 1861 to June 16, 1868 due to Civil War.
4. Vacancy from Mar. 4 to May 19, 1887. Jesse J. Finley had been appointed on Feb. 28, 1887, but never qualified. Pasco was elected, then appointed in 1893 by the governor while the legislature was not in session, then elected, then reappointed again in 1899, but he lost his reelection bid.
5. Elected to fill the vacancy for the term beginning Mar. 4, 1899. Reappointed in 1905, then reelected.
6. Elected for the term beginning on Jan. 3, 2019. Continued to serve as governor during interim.
7. Seat vacant from Jan. 21, 1861 to June 24, 1868 due to Civil War.
8. Vacancy from Mar. 4 to May 24, 1897. John A. Henderson was appointed by the governor on Mar. 6, 1897, but was not seated. Mallory was elected to fill the vacancy in 1897 when the state legislature met. He was subsequently appointed for term beginning Mar. 4, 1903, when the state legislature was not in session, and was then elected again in April 1903.
9. Appointed to the remainder of the term ending Jan. 3, 1975, having been elected to the term beginning on that day.
10. Appointed to the remainder of the term ending Jan. 3, 1981, having been elected to the term beginning on that day.
11. Resigned on January 20, 2025 to become secretary of State.
